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DNA evidence for different paternity

AI-generated conceptual cover showing fraternal twins and separate fertilization events; synthetic art, not a depiction of the people in the reported case. The original generation prompt and date were not preserved in the package.
Two newborn twins lying face to face; illustrative photo, not the Colombian case. [Original Commons file](https://commons.wikimedia.org/wiki/File:TwinsTwins.JPG); photographer MultipleParent~commonswiki; CC BY-SA 3.0 Unported or GFDL 1.2+; attribution: MultipleParent / Wikimedia Commons; no alteration.
Ultrasound showing dichorionic diamniotic twins in the fourth week after fertilization; illustrative, not from the reported Colombian case. [Wikimedia Commons file](https://commons.wikimedia.org/wiki/File:Dichorionic_diamniotic_twins.jpg), Entropy1963, own work, 24 March 2012, CC BY-SA 3.0. Credit the creator, link the license, indicate changes, and share adaptations under the same or compatible license.
Custom explanatory schematic created 2026-10-19 from the Colombian case described in sources.md. It shows two separate fertilizations as a simplified pathway, not the reported family or actual lab evidence; individual cases vary.
Diagram of sperm fertilizing a human egg and the early zygote stages; general biology illustration, not evidence about the reported case. [Wikimedia Commons file](https://commons.wikimedia.org/wiki/File:Egg_cell_fertilization_-_Zygote.png), Sciencia58, own graphic, 10 October 2019, CC0 1.0.

AI-generated conceptual cover showing fraternal twins and separate fertilization events; synthetic art, not a depiction of the people in the reported case. The original generation prompt and date were not preserved in the package.

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In 2020, researchers in Colombia described a case of fraternal twins in which one man had a 99.9999% probability of paternity for one twin but was excluded as the father of the other. The team examined autosomal and Y-chromosome STR markers; 14 of 17 Y markers and 14 of 21 autosomal STR markers did not match for the second twin. The 99.9999% probability belongs to this particular result, not to every paternity test.